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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
22/11/2002 |
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Data da última atualização: |
22/11/2002 |
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Autoria: |
TURZILLO, A. M.; CLAPPER, J. A.; MOSS, G. E.; NETT, T. M. |
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Título: |
Regulation of ovine GnRH receptor gene expression by progesterone and oestradiol. |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
Journal of Reproduction and Fertility, v. 113, n. 2, p. 251-256, 1998. |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this study was to determine whether progesterone prevents the stimulatory effects of oestradiol on GnRH receptor gene expression. In Expt 1, ewes were treated during the luteal phase (days 10-12 of the oestrous cycle) with either one or five subcutaneous implants containing oestradiol (n = 6 per group). Control ewes received no I treatment (n = 6). Anterior pituitary glands were collected 16 h after treatment with i oestradiol. Steady-state amounts of GnRH receptor mRNA were similar among all three I treatment groups despite increased circulating concentrations of oestradiol implanted ewes at the time of pituitary collection (4.3 +- 0.6 and 24.7 +- 2.6 pg ml-1 in ewes treated , with one or five implants, respectively, compared with 0.5 pg ml-1 in controls). Experiment 2 was designed to determine whether progesterone was the ovarian factor preventing the I stimulatory effects of oestradiol on. expression of the GnRH receptor gene in Expt 1. Twenty-five ewes were ovariectomized on day 6 or day 7 of the oestrous cycle and assigned to one of five treatment groups (n = 5 per group). Control ewes received no further treatment. Endogenous luteal phase concentrations of progesterone were replaced in three groups of ewes at the time of ovariectomy via intravaginal implants. Three days after ovariectomy, one group of progesterone-treated ewes received one oestradiol implant, while another group of progesterone-treated ewes received five oestradiol implants. An additional group was treated with five oestradiol implants only, and anterior pituitary glands were collected from alI ewes 16 h later. Compared with untreated ovariectomized ewes, treatment with progesterone alone did not affect amounts of GnRH receptor mRNA. In ewes treated with progesterone and either one or five oestradiol implants, steady-state amounts of GnRH receptor mRNA were increased twofold (P < 0.01). Treatment with oestradiol in the absence of progesterone increased amounts of GnRH receptor mRNA threefold (P < 0.001). These results provide evidence that the stimulatory effects of oestradiol on the expression of the GnRH receptor gene are prevented during the natural luteal phase in ewes. However, progesterone does not appear to act independently to mediate this effect. MenosThe objective of this study was to determine whether progesterone prevents the stimulatory effects of oestradiol on GnRH receptor gene expression. In Expt 1, ewes were treated during the luteal phase (days 10-12 of the oestrous cycle) with either one or five subcutaneous implants containing oestradiol (n = 6 per group). Control ewes received no I treatment (n = 6). Anterior pituitary glands were collected 16 h after treatment with i oestradiol. Steady-state amounts of GnRH receptor mRNA were similar among all three I treatment groups despite increased circulating concentrations of oestradiol implanted ewes at the time of pituitary collection (4.3 +- 0.6 and 24.7 +- 2.6 pg ml-1 in ewes treated , with one or five implants, respectively, compared with 0.5 pg ml-1 in controls). Experiment 2 was designed to determine whether progesterone was the ovarian factor preventing the I stimulatory effects of oestradiol on. expression of the GnRH receptor gene in Expt 1. Twenty-five ewes were ovariectomized on day 6 or day 7 of the oestrous cycle and assigned to one of five treatment groups (n = 5 per group). Control ewes received no further treatment. Endogenous luteal phase concentrations of progesterone were replaced in three groups of ewes at the time of ovariectomy via intravaginal implants. Three days after ovariectomy, one group of progesterone-treated ewes received one oestradiol implant, while another group of progesterone-treated ewes received five oestradiol implants. An additio... Mostrar Tudo |
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Palavras-Chave: |
GnRH; Receptor. |
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Thesagro: |
Endocrinologia; Estradiol; Hormônio; Ovino; Progesterona. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02932naa a2200241 a 4500
001 1528866
005 2002-11-22
008 1998 bl uuuu u00u1 u #d
100 1 $aTURZILLO, A. M.
245 $aRegulation of ovine GnRH receptor gene expression by progesterone and oestradiol.
260 $c1998
520 $aThe objective of this study was to determine whether progesterone prevents the stimulatory effects of oestradiol on GnRH receptor gene expression. In Expt 1, ewes were treated during the luteal phase (days 10-12 of the oestrous cycle) with either one or five subcutaneous implants containing oestradiol (n = 6 per group). Control ewes received no I treatment (n = 6). Anterior pituitary glands were collected 16 h after treatment with i oestradiol. Steady-state amounts of GnRH receptor mRNA were similar among all three I treatment groups despite increased circulating concentrations of oestradiol implanted ewes at the time of pituitary collection (4.3 +- 0.6 and 24.7 +- 2.6 pg ml-1 in ewes treated , with one or five implants, respectively, compared with 0.5 pg ml-1 in controls). Experiment 2 was designed to determine whether progesterone was the ovarian factor preventing the I stimulatory effects of oestradiol on. expression of the GnRH receptor gene in Expt 1. Twenty-five ewes were ovariectomized on day 6 or day 7 of the oestrous cycle and assigned to one of five treatment groups (n = 5 per group). Control ewes received no further treatment. Endogenous luteal phase concentrations of progesterone were replaced in three groups of ewes at the time of ovariectomy via intravaginal implants. Three days after ovariectomy, one group of progesterone-treated ewes received one oestradiol implant, while another group of progesterone-treated ewes received five oestradiol implants. An additional group was treated with five oestradiol implants only, and anterior pituitary glands were collected from alI ewes 16 h later. Compared with untreated ovariectomized ewes, treatment with progesterone alone did not affect amounts of GnRH receptor mRNA. In ewes treated with progesterone and either one or five oestradiol implants, steady-state amounts of GnRH receptor mRNA were increased twofold (P < 0.01). Treatment with oestradiol in the absence of progesterone increased amounts of GnRH receptor mRNA threefold (P < 0.001). These results provide evidence that the stimulatory effects of oestradiol on the expression of the GnRH receptor gene are prevented during the natural luteal phase in ewes. However, progesterone does not appear to act independently to mediate this effect.
650 $aEndocrinologia
650 $aEstradiol
650 $aHormônio
650 $aOvino
650 $aProgesterona
653 $aGnRH
653 $aReceptor
700 1 $aCLAPPER, J. A.
700 1 $aMOSS, G. E.
700 1 $aNETT, T. M.
773 $tJournal of Reproduction and Fertility$gv. 113, n. 2, p. 251-256, 1998.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pantanal. Para informações adicionais entre em contato com cpap.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
24/03/2008 |
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Data da última atualização: |
24/03/2008 |
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Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
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Autoria: |
PEREIRA, L. G. R.; GONÇALVES, L. C.; RODRIGUEZ, N. M.; TOMICH, T. R. |
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Afiliação: |
Luiz Gustavo Ribeiro Pereira, Embrapa Semi-Árido; Lúcio Carlos Gonçalves, UFMG; Norberto Mario Rodriguez, UFMG; Thierry Ribeiro Tomich, Embrapa Pantanal. |
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Título: |
Ensilaje de girasol como alternativa forrajera. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
In: JORNADA SOBRE PRODUCCIÓN Y UTILIZACIÓN DE ENSILAJES, 2007, Bahía Blanca. Un nuevo desafío para la producción regional. Bahía Blanca : Universidad Nacional del Sur, 2007. v. 1. p. 31-50. |
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Idioma: |
Português |
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Conteúdo: |
El girasol (Helianthus annuus L.) es una dicotiledónea anual adaptada a los climas
templado, tropical y subtropical. Su menor ciclo de producción, su capacidad en utilizar
el agua disponible en el suelo y la tolerancia a una amplia faja de temperaturas son
factores que han promovido el cultivo de girasol para la producción de ensilajes. |
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Palavras-Chave: |
Ensilaje; Forragera; Girasol. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00984naa a2200193 a 4500
001 1786491
005 2008-03-24
008 2007 bl --- 0-- u #d
100 1 $aPEREIRA, L. G. R.
245 $aEnsilaje de girasol como alternativa forrajera.
260 $c2007
520 $aEl girasol (Helianthus annuus L.) es una dicotiledónea anual adaptada a los climas templado, tropical y subtropical. Su menor ciclo de producción, su capacidad en utilizar el agua disponible en el suelo y la tolerancia a una amplia faja de temperaturas son factores que han promovido el cultivo de girasol para la producción de ensilajes.
653 $aEnsilaje
653 $aForragera
653 $aGirasol
700 1 $aGONÇALVES, L. C.
700 1 $aRODRIGUEZ, N. M.
700 1 $aTOMICH, T. R.
773 $tIn: JORNADA SOBRE PRODUCCIÓN Y UTILIZACIÓN DE ENSILAJES, 2007, Bahía Blanca. Un nuevo desafío para la producción regional. Bahía Blanca : Universidad Nacional del Sur, 2007.$gv. 1. p. 31-50.
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